Wednesday, November 27, 2019

SF-9 Lepidopteran Cells Essays - Benzofurans, Biochemistry Methods

SF-9 Lepidopteran Cells Essays - Benzofurans, Biochemistry Methods SF-9 Lepidopteran Cells PHM499 Research Project Supervisors: Dr. P. S. Pennefather, Dr. S. M. Ross Calcium transport study of SF-9 lepidopteran cells and bull frog sympathetic ganglion cells Kenny Yu Faculty of Pharmacy, University of Toronto, 19 Russell Street, Toronto, Ontario M5S 2S2 ABSTRACT The intracellular calcium level and the calcium efflux of the bull-frog sympathetic ganglion cells (BSG) and the SF-9 lepidopteran ovarian cells were investigated using a calcium-sensitive fluorescence probe fura-2. It was found that the intracellular calcium levels were 58.2 and 44.7 nM for the BSG cells and SF-9 cells respectively. The calcium effluxes following zero calcium solution were 2.02 and 1.33 fmolecm-2s-1 for the BSG cells and SF-9 cells. The calcium effluxes following sodium orthovanadate (Na2VO4) in zero calcium solution were 6.00 and 0.80 fmolecm-2s-1 for the BSG cells and the SF-9 cells. The SF-9 cells also lost the ability to extrude intracellular calcium after 2-3 applications of Na2VO4 while the BSG cells showed no apparent lost of calcium extruding abilities for up to 4 applications of Na2VO4. INTRODUCTION Spodoptera frugiperda clone 9 (SF-9) cells are a cultured insect cell line derived from the butterfly ovarian tissue. SF-9 cells are used by molecular biologists for the studies of gene expression and protein processing (Luckow and Summers, 1988). However, there is not much known about these cells' basic biophysiology. Since calcium is involved in many cells' activities such as acting as a secondary messenger, it is important for cells to control their intracellular calcium level. This study was aimed toward looking at the some of the basic properties of the SF-9 cells such as resting calcium concentration and rate of calcium extrusion after being calcium level being raised by an ionophore 4-bromo-A23187. The effect of sodium orthovanadate (an active transport inhibitor) on calcium extrusion was also looked at. Microspectrofluorescence techniques and the calcium-sensitive probe fura-2 were used to measure the intracellular calcium concentration of these cells. In addition, the BSG ce lls were used to compare with the SF-9 cells for the parameters that were studied. It was found that the SF-9 cells appeared to have a calcium concentration similar to the BSG cells. Moreover, the calcium extrusion rates of both cell types with no Na2VO4 added seemed to the same. However, due to insufficient data, the effects of Na2VO4 could not be statistically analyzed. From the data available, it suggested that the BSG cells' rate of calcium extrusion was enhanced by the Na2VO4 and was greater than the SF-9 cells. It was more important to note that the calcium extruding capabilities of the SF-9 cell seemed to impaired after two to three applications of Na2VO4 but it had apparent effects on the BSG cells even up to 4 applications. After obtaining these basic parameters, many questions raised such as how does the SF-9 cells extrude their calcium and why the Na2VO4 affected the calcium efflux for the SF-9 cells but not the BSG cells? The SF-9 cells may have a calcium pump or exchanger to extrude their calcium and they may be very sensitive to the ATP (adenosine 3'-triphosphate) supply. This was apparently different from the BSG cells' since their calcium extrusion were not affected by the Na2VO4.. It may be useful to find the mechanism(s) of the actions of Na2VO4 on the SF-9 cells because it may find possible applications in agriculture such as pest control. MATERIALS AND METHODS Chemicals and solutions 4-bromo-A23187 and Fura-2/AM were purchased from Molecular Probes (Eugene, OR). Na2VO4 was purchased from Alomone Lab (Jerusalem, Israel). Dimethyl sulfoxide (DMSO) was obtained from J. T. Baker Inc. (Phillipsburg, NJ). All other reagents were obtained from Sigma (St. Louis, MO). The normal Ringer's solution (NRS) contained (mM): 125 NaCl, 5.0 KCl, 2.0 CaCl2, 1.0 MgSO4, 10.0 glucose, 10.0 N-[2-hydroxyethyl] piperazine-N'-[2-ethanesulfonic acid] (HEPES). The calcium free Ringer solution (0CaNRS) is the same as the NRS except CaCl2 was substituted with 2.0 mM ethylene glycol-bis(b-aminoehtyl) ether N,N,N',N'-tetraacetic acid (EGTA). Fura-2/AM solution was prepared as follows: a stock solution of 1mM fura-2/AM in DMSO was diluted 1:500 in NRS containing 2% bovine albumin. It was then sonicated for 10 minutes. It was then kept frozen until the day of the experiment. 20 SYMBOL 109 f "Symbol"M 4-bromo-A23187 solution was prepared by diluting a stock of 5mM 4-bromo-A23187 in DMSO 1:250 with NRS. Na2VO4 solution

Saturday, November 23, 2019

Start your website in 3 steps

Start your website in 3 steps How to Start your Website from Scratch Introducing: a step-by-step guide on how to make a website from the ground up without a degree in programming or any knowledge of coding and design. Tune in! Get a domain name for your website First thing first, you need to come up with a name for your site that won’t be too tricky to find for your target audience. Usually, the cost for domain names starts from $10 and gets as high as $35 if you buy them at a certified registrar. Once you pay for it, you get the right to own your name for a year, and if you want to secure your ownership for the next years to come, you pay the same fee per annum. But if this sounds crazy to you, there’s also an option to get your domain name for free. When it comes to making up a good name, most of them can be already taken, especially in the product industry. So if you really want to be allocated a particular name, but it’s already reserved, try to add â€Å"-† or digits to it and see if it is available. Choose a web host Selecting a web host is basically like renting an office for business but on the Internet; it’s a platform that connects you to other computers on the Internet and lets them find you. Before you invest your money in the host, consider which one will fit your needs the best, a free web host, or a commercial one; they generally differ but have similar overlaps. Let’s take a closer look at both options. A free web host is unmistakably the best solution for those who are on the budget and looking for cheaper deals. However, there’s a price you pay for getting your host for free. Pros: FREE Cons: Impose advertising Limited web space Single site builder option File type size limitations Questionable reliability and speed Limited data transfer Here’s what’s the deal with commercial web hosts - they are far more reliable, but they also can be surprisingly tricky to work with. Pros: Reliable Fast Near-Unlimited Bandwidth More Web Space Technical Support Support for Various Scripts SSL Option Cons: Hefty price tag Create Design After you’ve saddled your website with a domain name and host provider, your next step, not as unimportant, is to make a smashing design, or at least the one that your clients will be pleased with. The easiest way to complete this step would be to hire a decent web designer, but if it’s not what you can afford, then keep on reading! As a beginner, you just need to get something out onto your page to at least frame your website. Later, you can fine-tune your design or redo the whole thing for the sake of your clients. To start making your custom design, you can choose one of the WYSIWYG ("What You See Is What You Get") web editors. The range is huge; ones are great if you are using Windows PC, others have a better view of mobile design and guide you through this process. Conclusion Cost-wise starting up a website isn’t such an expensive thing until you know how to arrange a good deal. While the research will still take much of your time - the actual amount of time you’ll spend setting up your website can be less than 1 hour!

Thursday, November 21, 2019

Near Failure at Nagasaki Essay Example | Topics and Well Written Essays - 750 words

Near Failure at Nagasaki - Essay Example The same problem is observed relating to Sweeney’s relation to flight engineer Kuharek such that when the latter firstly identified the lack of proper fuel in the tanks, Sweeney declined from communicating with Kuharek and moved over to Tibbets for gaining advice. Sweeney also reflected lack of self-confidence that is a significant necessity for a leader to govern combat operations. Firstly owing to his lack of proper knowledge of the combat he was highly dependent on the knowledge and expertise of Ashworth such that the latter exerted significant influence on him. Sweeney’s lack of self confidence owing to his limited knowledge again required Ashworth to help him identify and reach the target. Sweeney also lacked self-confidence in dropping the bomb effectively on the target for which he aptly depended on Beahan, the bombardier incorporated in the flight operations. Similarly other non-leadership qualities are also evident relating to Sweeney in which it is found that how Sweeney shifted the responsibility for the Nagasaki Mission’s fault to the shoulder of Hopkins. ... ation to be rendered to Hopkins regarding the position of the instrument aircraft which in turn deferred the operations much and made things complicated. Thirdly owing to the extra time spent by Sweeney further deferred his operations in being able to find the effective target of Kokura. This only required Sweeney to counter shift in his original plans. Thirdly inability of Sweeney to find both the effective and alternative targets and his dependency on Ashworth delayed the operations much creating threat of excess consumption of fuel. Fourthly owing to the incapability of taking decisions in a fast and timely fashion and dependence on his followers made Sweeney counter excess time loss in dropping the bomb over the target effectively. He shifted between dependency on the radar or on visual sighting to drop the bomb. Fifthly, Sweeney acted on a strange note on going on continuously circling above the target, Kokura when it was clear to be bombed that further deferred the operations. Evaluation made later on reflects that Sweeney had lost around one and a half hours in his failure to take decisions on time leading only to circling over the target a number of times. This failure to calculate the time required for the operation to be completed made Sweeney suffer from the threat of loss of required fuel to charge for the alternate targets. This continuous and unused circling over the rendezvous point made Sweeney also counter the threat of proper landing. The time being spent in an unused fashion thus triggered the need for Sweeney to prepare for a harsh landing than preparing for a crash landing. Sweeney’s failure to take decisions in a timely fashion also made him fail in catching sight of the instrument-carrying carrier. His failure to catch up with the

Wednesday, November 20, 2019

Counterinsurgency Essay Example | Topics and Well Written Essays - 1250 words

Counterinsurgency - Essay Example Accordingly, the primary focus should be to improve the quality of the police and other security forces, strengthen government institutions, and separated the populace from the insurgents. Contemporary counterinsurgency methodologies introduced in the Philippines, Malaya, Algeria and Vietnam prove when the government accomplished these tasks, it defused the insurgency's political and ideological premise, discredited their cause, and created a political environment unsuitable for an insurgency to thrive. DISCUSSION: Intelligence reports show clashes between Taliban and coalition forces have increased significantly in 2008, highlighting the Taliban's resurgence and complicating NATO efforts to stabilize the country. Taliban, Hekmatyar, and Haqqani militants have expanded their influence in rural regions where NATO/ISAF and the Afghan government cannot provide sufficient security. Violent attacks have tripled in these areas - particularly against civilian non-combatants perceived to be in support of the government. Consequently, the U.S. planners must convince NATO and commanders to employ specific counterinsurgency approaches to reverse these trends. 1. Secure the Afghan-Pakistan border. ... Thus far, US/NATO strike operations along the border and inside Afghanistan have not curtailed militant force infiltrations and security forces have been unable to pursue retreating insurgents across the border. In order prevent these incursions, a more audacious containment strategy must be implemented. Measures include increasing security force levels in select border regions, formalizing intelligence cooperation activities with Pakistan, and erecting barriers along major infiltration corridors. First, NATO must expand the International Security Assistance Force (ISAF), Afghan National Police (ANP), and Afghan National Army (ANA) presence in the remote border regions where infiltrations and armed attacks most often occur. Diligent law enforcement activities should be the primary focus in populated areas and villages to disrupt support sanctuaries and networks logistics networks. ANA forces should occupy security checkpoints and border encampments to interdict hostile incursions. In the meantime Afghan and Pakistani officials formally demarcate the Durand Line by establishing a mutually recognized border, then erect a series of defensive fences along known infiltration corridors to deny militants access into Afghanistan. Technology based surveillance systems and interdiction platforms must be employed in tandem with physical structures. French counterinsurgents successfully employed similar fencing startegy in Algeria when they built the Mortice Line to contain the Front de Liberation Nationale (FLN) insurgents. Within a year of construction, the eight foot electrical fence proved to be a decisive counterinsurgency additive. The combination of static defenses and mobile border forces had killed over 6,000 would-be intruders and intercepted

Sunday, November 17, 2019

Cellular Respiration Essay Example for Free

Cellular Respiration Essay Answer the following questions: Cellular respiration: †¢ What is cellular respiration and what are its three stages? Cellular respiration is a way cells store food and energy, a catabolic pathway for the production of adenosine triphosphate (ATP). The cellular respiration happens in both eukaryotic and prokaryotic cells. The three stages are Glycolysis, Citric cycle, and electron transport. †¢ What is the role of glycolysis? Include the reactants and the products. Where does it occur? Glycolysis splits the sugar that goes in to the cell. Then in converts in to energy the cell need. It does not need oxygen to occur. †¢ What is the role of the citric acid cycle? Include the reactants and the products. Where does it occur? Citric acid occurs after glycolysis process, high energy electrons are produced. It occurs only when oxygen is present but does not always use it. †¢ What is the role of the electron transport system? Include the reactants and the products. Where does it occur? Electron transportation system requires oxygen. It’s a series of electrons carriers in the membrane of the mitochondria. Photosynthesis: †¢ What is the overall goal of photosynthesis? Photosynthesis is a process whereby plants, algae and bacteria convert light energy in to chemical energy, using carbon dioxide and water. †¢ Because photosynthesis only occurs in plants, why is it essential to animal life? Photosynthesis is important for animals because the plants produce the sugar they need as a vital nutrient for the animals. †¢ What is the role of the light reactions? Include the reactants and the products. Where does it occur? The reactants of light-dependent reactions in photosynthesis are H20 (water), ADP, and NADP+. The products of light-dependent pathways of photosynthesis are Oxygen, ATP, and NADPH. The reactants of light-independent reactions are ATP, NADPH, and Carbon Dioxide. The main purpose of the light independent reaction is to produce glucose. Rate This Answer What is the role of the Calvin cycle? Include the reactants and the products. Where does it occur? Summary: †¢ Explain how photosynthesis and cellular respiration are linked within ecosystems. The link between photosynthesis and cellular respiration is an inverse relationship; both are opposites of each other. Photosynthesis is the process by which carbon dioxide is converted into organic compounds from sunlight. The most frequent compound is sugar. †¢ Visit the NASA website (http://data.giss.nasa.gov/gistemp/graphs/) and research global temperature changes. How has global warming affected overall temperatures? What effects do cellular respiration and photosynthesis have on global warming? References. 1. http://www.biolib.cz/en/main 2. UnversityofPhoenix(2011)Photosentisys.p109

Friday, November 15, 2019

The Effective Decision Essay -- GCSE Business Marketing Coursework

The Effective Decision The Effective Decision - The Function of the Chief Executive At 60, John Neyland, the company president, decided he would retire before the mandatory retirement age of 65. He did not reveal his decision to anyone until he reached 62, and at this time he confided to his best friend and the most powerful board member that he would retire imminently. Mr. Neyland proposed that Bill Strong, Vice President, Administration, a very able and experienced executive, succeed him as president. Mr. Neyland's friend vehemently opposed Bill Strong's candidacy, and forcefully argued that Margaret Wetherall, vice president of manufacturing, was the best qualified to be the new president. This case presents a situation where the decision-making process has completely failed. The selection of the president is one of the most important decisions a board of directors makes. Not only does a president have an enormous impact on the fortunes of a company, but the very process by which the executive is picked influences the way employees, investors, and other constituencies view the company and its leadership. One of the board's most critical roles is to ensure the presence of an effective management development program for the whole enterprise. While the CEO (in most firms, the president is also the CEO), is the person managing the program, the board needs to play an active oversight role to ensure that the program is in place and is working effectively. Considering that Mr. Neyland was approaching the mandatory retirement age, and that a significant difference in opinion between Mr. Neyland and the most powerful board member as to who should be the new president, it is clear that the board (the president is almost always a board member) was extremely derelict in its duties. The decision-making process was greatly undermined, with huge ramifications for the organization. In the Japanese way of decision-making, the single most important element in solving such problems is defining the question. Because the Japanese system is very time consuming and involves many participants from various functions within the organization, the Japanese system is suited to big decisions. A change is president is one of the most crucial events in the life of a company, and it is an event in which the board of directors plays a central role. Because the ne... ...ns by consensus, and they have developed a systematic decision-making process. The critical first step in the Japanese decision-making system is to define the problem and then proceed through well-defined stages to arrive at an effective decision. For example, the Japanese flush out various opinions without any discussion of the answer. The Japanese focus on exploring and debating the merits of alternatives, rather than on the optimal solution. The process includes all parties that are affected by the decision. When a consensus is reached, the decision can be easily implemented because people implementing the decision were intimately involved in the decision-making process. The disagreement between Mr. Neyland and the board member regarding who should succeed Mr. Neyland has sabotaged the effective decision-making process. It is highly unlikely that the next president will be the "best" candidate, and politics will compromise the integrity of the decision process. Naturally, there are enormous implications for the economic health of the organization. American and European managers often make poor decisions, and the consequences can be devastating for their organizations.

Tuesday, November 12, 2019

To what extent can Reagan’s electoral victory in 1980 be put down to the rise of the new right?

1980's America saw a boom in a new group of hard-line Christians; known as the ‘new right', a powerful group of republican evangelicals set on restoring the American morals of old (with somewhat a very archaic mindset for example no equality for homosexuals etc. ) This group took a very strong liking to Reagan and his strong Christian moral conservatism and thus earned him millions of votes in the election of 1980. Was Reagan's victory largely down to the rise of the new right? Or were there other more prominent factors, which lead to Reagan's victory? In 1980s America TV could be used as a powerful political tool, 67% of American's received 100% of all there news from the television, this clearly showing if televised speeches, debate and propaganda were used correctly it could be a direct, simple and powerful method to connect with the people- winning over the votes of millions of American's. Reagan executed all his televised appearances like a professional (he was an ex-Hollywood ‘star' which definitely helped immensely,) ‘he could read an autocue like a professional'. Also his personal traits were key – portraying himself as a ‘physically attractive and charming man who was gracious and polite' this again helped him as all those were key and made Reagan a much more likeable person. Furthermore, Reagan also worked with general electric in the 60's where he was in charge of the TV shows; he also gained valuable electioneering skills during the job, as he had to meet thousands of people daily, also giving unrehearsed speeches to hundreds. The job handed him with a perfect chance to groom his campaigning skills to a respectable audience of 700,000, which was tiny compared to the people of America but still a good start, where he learnt how to be a people person and how to work the TV. In contrast to this his main opponent – incumbent president, Jimmy Carter was quite the opposite to the charming, attractive Reagan. He delivered his ‘crisis of confidence' speech where he found it easy to find problems but couldn't seem to deliver any solutions. This again showed Reagan's superiority in these areas where he delivered short and direct targets such as reviving American strength in the world once again. This again gained him popularity as it gave the people something to look forward to and it showed he meant business unlike the passive carter. Carter, having completed one term in power had done next to nothing useful, he became know as a man who would deal with problems when they came rather than trying to predict them and stop them from happening- not what you want for the worlds most powerful man. During his presidency he grew more foolish and weak in the eys of America. Almost nothing positive happened during his presidency; America's ditente with the USSR ended, there was an energy crisis. Also his failure of a brother somewhat cast negativity towards him making him look more foolish and weak. His ‘crisis of confidence' speech was completely crazy he informed America of its problems including a lack of leadership- ‘now all we need is leadership' a mildly retarded thing to say, as he was ‘the' leader of America, and still didn't give any solutions to the problems he presented. It was clear that nothing had changed for the good from Nixon's presidency. The economy was still stuck in the stagflation caused by Nixon, carter had done nothing but worsen it. Reagan used carters ‘nothing' presidency where almost nothing was done, to his advantage- he promised to renew prosperity by restoring the economy through ‘reaganomics' where there would be lower taxes and less regulation – curing the stagflation. No one knew it would work but it was a lot more than carter offered. Reagan also had vast amount of political training from being an active trade unionist where he established himself as a strong anti-communist (again extremely popular with the lingering cold war and also very popular with the new right who wanted a return of the traditional morals) and also the job was said to help ‘gain an apprenticeship in negotiating, to develop an instinct for when to ‘hang-tough' and when to cut a deal' by a political analyst- which would clearly help him become a successful president. He was also the governor of California from 1967-75, which was a massive success, and he managed to make California the seventh richest ‘country' in the world- showing he knew how to work economics, which is what America vitally needed! He also had the experience of running for president as he had attempted on two other occasions. All this political experience would be priceless for his campaign. Reagan was also extremely conservative which also gained him lots of votes- as he stood for mostly traditional values such as; no abortion, pornography, drugs, and equality for homosexuals. He was also a strong evangelical Christian, which initially gained him the support of the new right and with it groups such as the ‘moral majority' as they had the backing from Reagan and they believed he was going to bring American morals back. He also gained support from the Neo conservative's traditionalists and anti feminists, also vitally he managed to get the support of the born again Christians even though carter tried his hardest to gain there support being a born again Christian too, Reagan managed to do so with his conservative ideology. The new right was essential for his campaign, as Reagan had such radical ideas many would have seen him as crazy and never given him a chance – comparing him to the extremist Barry Goldwater. The new right rather embraced his ideas as they fitted in well with what they wanted. Reagan was extremely lucky that this spark in Christianity coincided with this electoral campaign as if he failed it would most probably be the end of him as he was aging and many were already hesitant to elect such an old man. In conclusion, ii feel it is very clear that the rise of the new right played a very significant role in Reagan's ascendancy to power, and without a doubt without this support he probably couldn't win as it allowed him to create a base of support from which he could build around and add onto. However, I believe that there were other more influencing factors which lead to his presidency such as his political ingeniousness particularly offering an intelligent solution to the stagflation suppressing the country, as well as the mans personal characteristics such as his personal charm and talent in front of the TV which allowed him to manipulate millions as they could see it with there own eyes that he was an astute leader. But, from the election results we see such a narrow win on Reagan's side this even so when millions of democrats didn't even vote, I believe that this shows us that Reagan won largely due to the failures of Carter as even though he was such a useless leader who did next to nothing he still managed to almost win the elections, furthermore he still came so close even with a large percent of his ‘party' boycotting the election- showing carter didn't have a very large support base, and if he did have decent opposition Reagan could have lost by a landslide.

Sunday, November 10, 2019

Hot Wire Laboratory

THE UNIVERISTY OF MANCHESTER SCHOOL OF MECHANICAL, AEROSPACE AND CIVIL ENGINEERING LABORATORY REPORT INSTRUMENTATION AND MEASUREMENT VORTEX SHEDDING FROM A CYLINDER & DATA ACQUISITION NAME:MANISH PITROLA STUDENT ID:75050320 COURSE:MEng MECHANICAL ENGINEERING DUE DATE:27TH NOVEMBER 2012 1) What are the main advantages and disadvantages of using a hotwire to measure flow velocities?There are many advantages and disadvantages of using a hotwire to measure flow velocities, one of the main advantages is the hotwire produces a continuous analogue output of the velocity at a particular point, and hence information about the velocity can be obtained for any specific time. Another advantage of using a hotwire anemometer is the ability to follow fluctuating velocities to a high accuracy. Also another advantage of using a hotwire anemometer is the sensor is able to relate the voltage and the velocity using hotwire theory. However even though hotwire anemometer is an adequate tool to obtain data it has its drawbacks. Read this  Respiratory ActivityOne disadvantage of using a hotwire is that it has to be calibrated due to the theory not coinciding with actual data and the hotwire can only obtain the magnitude of the flow and not the direction. Another disadvantage of using a hotwire is the unsystematic effects that occur such as contamination and probe vibration. Some systematic effects that affect the data are the ambient temperatures and eddy shedding from the wire. One of the main disadvantages of using a hotwire is the output depends on both velocity and temperature, so when the temperature of a fluid increases the measured velocity obtained are too low and adjustment is required. ) Why is setting the correct sampling rate important in digital data acquisition? What experimental parameters or requirements can be used to establish the optimum sampling rate? What may happen if the wrong sampling rate is used? Using the correct sampling rate is important because if the incorrect sampling rate i s used some aliasing effects may occur, presenting insufficient data where important data is ignored if the sampling rate is below the optimum, and if the sampling rate is above the optimum more accurate data is obtained which carries the same trend as the optimum with few distortion which are not required.This can cause inadequacy of the data, where recording is not frequent enough or too frequent. The optimum sampling rate can be established using the Nyquist theory which states that the maximum measures frequency is half the sampling frequency, however the bandwidth of the signal needs to considered, the rule for obtaining the sampling frequency of any probe must be at least 2. 5 times greater than the maximum frequency present. 3) Show how the sampling rate was determined for this experiment.What was the sampling rate? For a flow around a cylinder an empirical relation between the vortex shedding frequency and Reynolds number (Re) is used to find the sampling rate. The relations hip below is used to find the frequency in the flow where the Strouhal number is 0. 2, diameter (d) is 15mm and the free stream velocity (U0) is 10m/s. St=fdU0=0. 1981-19. 7Re? 0. 2 Then by simple algebraic rearranging the frequency is found to be 133. 3Hz. Therefore the maximum frequency experienced is 2f = 2*133. 3 = 266. 6Hz.To obtain the optimum sampling frequency we simply by using Nyquist theory multiply the maximum frequency by 2. 5 providing an optimum sampling rate of 666. 5Hz. The values for the sampling rate were taken as 330Hz, 660Hz and 1320Hz for experimental purposes to study the over and under sampling of data. 4) In the experiment the hotwire was calibrated in terms of velocity vs (E-E0)2. Plot out the calibrations for U = B((E-E0)2)n and the various polynomials. Compare the different lines. Which is the best to use? Figure [ 1 ] Figure [ 2 ] Figure [ 3 ]Figure [ 4 ] From the above graphs is can be seen that the best calibration to use is the cubic calibration (figu re 2) as this fits the actual velocity line more accurately. 5) If the velocity higher than the ones calibrated foer was measured, which calibration is likely to give the best extrapolated data? Figure [ 5 ] Figure [ 6 ] Figure [ 7 ] Figure [ 8 ] From the above graphs it can be seen that the worse extrapolated data is found using the quartic calibration and the best extrapolated data can be found using the linear calibration of A([V-Vo]^2)^n.Also higher order polynomial extrapolation can produce invalid values and as a result the error will magnify as high order of polynomials are used, so therefore the linear relationship is recommended. 6) In a fast Fourier transform (FFT) the data in the time domain is converted to the equivalent data in the frequency domain. The original data can therefore be considered as the sum of a series of sine waves of regularly spaced frequencies, with different magnitudes and phases. How is the frequency interval in the FFT determined? How can the frequ ency interval in an FFT be reduced?What impact could this have on an experiment? The frequency interval can be obtained by dividing the sampling rate by the number of samples used. For 660Hz the number of samples is 1024, so therefore the frequency interval is 660/1024 = 0. 6445. The frequency intervals can be reduced by increasing the number of samples used; this is advantageous as it gives a more accurate representation of the original signal. 7) Considering the FFT data, what can be done in an experiment to isolate genuine signals from random fluctuations in the data? Give an example of this in graphical form.Figure [ 9 ] Figure [ 10 ] From figure 9 it can be seen that the peak is unobtainable as the data is very noisy which could be due to disturbances. However this can be overcome by averaging the FFT which allows us to easily identify peaks which can be seen from figure 10. 8) In this experiment, why are 2 frequency peaks seen on the FFT when the hotwire is near the centre lin e? 2 frequency peaks can be seen on the FFT at the centreline due to the 2 vortices induced by the cylinder but as you move away from the centre line only one of the vortices is predominant.The two peaks occur at 129Hz and 250Hz. 9) With increasing distance from the centreline, how does the FFT distribution change? Include graphs to illustrate this for various locations across the wake. From the below figures it can be seen that as you move away from the centre line the peaks in the FFT distribution disappear. Figure [ 11 ] Figure [ 12 ] Figure [ 13 ] Figure [ 14 ] Figure [ 15 ] Figure [ 16 ] 10) Plot the probability distribution histograms of velocity for various positions across the wake.What does the histogram show and how can the variation in the histograms be explained in terms of the properties of the flow? Figure [ 17 ] Figure [ 18 ] Figure [ 19 ] Figure [ 20 ] Figure [ 21 ] Figure [ 22 ] By comparing the above probability distribution figures it can be seen that with distanc e away from the centreline the flow velocity develops a more uniform velocity. It can be seen that within the 40mm distance away from the centreline, the probability distribution of the velocity produces wide distribution of velocities; this is due to the various velocities inside the wake and turbulence.For distance more than 40mm away the probability distribution of velocity becomes more uniform, which implies the vortices play no role in affecting the flow at these distances away from the centreline. It can also be seen that the flow speed at these distances increases as the flow diverges and accelerates around the cylinder. 11) Plot a graph showing the variation of mean velocity, RMS velocity and turbulence intensity with distance across the wake. What physical phenomena in the flow are causing the distribution to be the shape they are?What do the results say about the size of the wake compared to the size of the cylinder? Figure [ 23 ] Figure [ 24 ] Figure [ 25 ] The vortices i n the flow cause turbulence to occur behind the cylinder which causes the distributions to change. It can be seen from figure 23 that the velocity changes instantaneously as you move away from the centreline, it can also be observed that from 45mm away and more the velocity start to become more uniform and fluctuate around the free stream velocity. From figure 25 and 25 from 45mm and onwards the RMS and RTI decrease.From the above graphs it can be deduced that the size of the wake is 45mm from the centreline or a total width of 90mm, which is 6 times the diameter of the cylinder. 12) What are the major sources of error likely to be in this experiment? Try and give a numerical estimate to the possible error(s) in the data. Some of the likely sources of error that may occur during this experiment are the calibration process as the hotwire was only calibrated at the centreline and as the hotwire was lowered using screw mechanism which it not totally accurate, there was no calibration o f the at the new position.Another source of error can be due to pressure fluctuations, and due to the velocity being measured using the pressure differences, these fluctuation can cause the velocity to vary. Another source of error could be the assumption of the flow being 2-d as turbulence is a 3-d. To calculate the error, I used the measured velocity table and the theoretical linear calibration velocity. Taking the average error, the percentage error in the experimental data was 5. 8%. Within a range Can not measure supersonic velocities Hot Wire Laboratory THE UNIVERISTY OF MANCHESTER SCHOOL OF MECHANICAL, AEROSPACE AND CIVIL ENGINEERING LABORATORY REPORT INSTRUMENTATION AND MEASUREMENT VORTEX SHEDDING FROM A CYLINDER & DATA ACQUISITION NAME:MANISH PITROLA STUDENT ID:75050320 COURSE:MEng MECHANICAL ENGINEERING DUE DATE:27TH NOVEMBER 2012 1) What are the main advantages and disadvantages of using a hotwire to measure flow velocities?There are many advantages and disadvantages of using a hotwire to measure flow velocities, one of the main advantages is the hotwire produces a continuous analogue output of the velocity at a particular point, and hence information about the velocity can be obtained for any specific time. Another advantage of using a hotwire anemometer is the ability to follow fluctuating velocities to a high accuracy. Also another advantage of using a hotwire anemometer is the sensor is able to relate the voltage and the velocity using hotwire theory. However even though hotwire anemometer is an adequate tool to obtain data it has its drawbacks. Read this  Respiratory ActivityOne disadvantage of using a hotwire is that it has to be calibrated due to the theory not coinciding with actual data and the hotwire can only obtain the magnitude of the flow and not the direction. Another disadvantage of using a hotwire is the unsystematic effects that occur such as contamination and probe vibration. Some systematic effects that affect the data are the ambient temperatures and eddy shedding from the wire. One of the main disadvantages of using a hotwire is the output depends on both velocity and temperature, so when the temperature of a fluid increases the measured velocity obtained are too low and adjustment is required. ) Why is setting the correct sampling rate important in digital data acquisition? What experimental parameters or requirements can be used to establish the optimum sampling rate? What may happen if the wrong sampling rate is used? Using the correct sampling rate is important because if the incorrect sampling rate i s used some aliasing effects may occur, presenting insufficient data where important data is ignored if the sampling rate is below the optimum, and if the sampling rate is above the optimum more accurate data is obtained which carries the same trend as the optimum with few distortion which are not required.This can cause inadequacy of the data, where recording is not frequent enough or too frequent. The optimum sampling rate can be established using the Nyquist theory which states that the maximum measures frequency is half the sampling frequency, however the bandwidth of the signal needs to considered, the rule for obtaining the sampling frequency of any probe must be at least 2. 5 times greater than the maximum frequency present. 3) Show how the sampling rate was determined for this experiment.What was the sampling rate? For a flow around a cylinder an empirical relation between the vortex shedding frequency and Reynolds number (Re) is used to find the sampling rate. The relations hip below is used to find the frequency in the flow where the Strouhal number is 0. 2, diameter (d) is 15mm and the free stream velocity (U0) is 10m/s. St=fdU0=0. 1981-19. 7Re? 0. 2 Then by simple algebraic rearranging the frequency is found to be 133. 3Hz. Therefore the maximum frequency experienced is 2f = 2*133. 3 = 266. 6Hz.To obtain the optimum sampling frequency we simply by using Nyquist theory multiply the maximum frequency by 2. 5 providing an optimum sampling rate of 666. 5Hz. The values for the sampling rate were taken as 330Hz, 660Hz and 1320Hz for experimental purposes to study the over and under sampling of data. 4) In the experiment the hotwire was calibrated in terms of velocity vs (E-E0)2. Plot out the calibrations for U = B((E-E0)2)n and the various polynomials. Compare the different lines. Which is the best to use? Figure [ 1 ] Figure [ 2 ] Figure [ 3 ]Figure [ 4 ] From the above graphs is can be seen that the best calibration to use is the cubic calibration (figu re 2) as this fits the actual velocity line more accurately. 5) If the velocity higher than the ones calibrated foer was measured, which calibration is likely to give the best extrapolated data? Figure [ 5 ] Figure [ 6 ] Figure [ 7 ] Figure [ 8 ] From the above graphs it can be seen that the worse extrapolated data is found using the quartic calibration and the best extrapolated data can be found using the linear calibration of A([V-Vo]^2)^n.Also higher order polynomial extrapolation can produce invalid values and as a result the error will magnify as high order of polynomials are used, so therefore the linear relationship is recommended. 6) In a fast Fourier transform (FFT) the data in the time domain is converted to the equivalent data in the frequency domain. The original data can therefore be considered as the sum of a series of sine waves of regularly spaced frequencies, with different magnitudes and phases. How is the frequency interval in the FFT determined? How can the frequ ency interval in an FFT be reduced?What impact could this have on an experiment? The frequency interval can be obtained by dividing the sampling rate by the number of samples used. For 660Hz the number of samples is 1024, so therefore the frequency interval is 660/1024 = 0. 6445. The frequency intervals can be reduced by increasing the number of samples used; this is advantageous as it gives a more accurate representation of the original signal. 7) Considering the FFT data, what can be done in an experiment to isolate genuine signals from random fluctuations in the data? Give an example of this in graphical form.Figure [ 9 ] Figure [ 10 ] From figure 9 it can be seen that the peak is unobtainable as the data is very noisy which could be due to disturbances. However this can be overcome by averaging the FFT which allows us to easily identify peaks which can be seen from figure 10. 8) In this experiment, why are 2 frequency peaks seen on the FFT when the hotwire is near the centre lin e? 2 frequency peaks can be seen on the FFT at the centreline due to the 2 vortices induced by the cylinder but as you move away from the centre line only one of the vortices is predominant.The two peaks occur at 129Hz and 250Hz. 9) With increasing distance from the centreline, how does the FFT distribution change? Include graphs to illustrate this for various locations across the wake. From the below figures it can be seen that as you move away from the centre line the peaks in the FFT distribution disappear. Figure [ 11 ] Figure [ 12 ] Figure [ 13 ] Figure [ 14 ] Figure [ 15 ] Figure [ 16 ] 10) Plot the probability distribution histograms of velocity for various positions across the wake.What does the histogram show and how can the variation in the histograms be explained in terms of the properties of the flow? Figure [ 17 ] Figure [ 18 ] Figure [ 19 ] Figure [ 20 ] Figure [ 21 ] Figure [ 22 ] By comparing the above probability distribution figures it can be seen that with distanc e away from the centreline the flow velocity develops a more uniform velocity. It can be seen that within the 40mm distance away from the centreline, the probability distribution of the velocity produces wide distribution of velocities; this is due to the various velocities inside the wake and turbulence.For distance more than 40mm away the probability distribution of velocity becomes more uniform, which implies the vortices play no role in affecting the flow at these distances away from the centreline. It can also be seen that the flow speed at these distances increases as the flow diverges and accelerates around the cylinder. 11) Plot a graph showing the variation of mean velocity, RMS velocity and turbulence intensity with distance across the wake. What physical phenomena in the flow are causing the distribution to be the shape they are?What do the results say about the size of the wake compared to the size of the cylinder? Figure [ 23 ] Figure [ 24 ] Figure [ 25 ] The vortices i n the flow cause turbulence to occur behind the cylinder which causes the distributions to change. It can be seen from figure 23 that the velocity changes instantaneously as you move away from the centreline, it can also be observed that from 45mm away and more the velocity start to become more uniform and fluctuate around the free stream velocity. From figure 25 and 25 from 45mm and onwards the RMS and RTI decrease.From the above graphs it can be deduced that the size of the wake is 45mm from the centreline or a total width of 90mm, which is 6 times the diameter of the cylinder. 12) What are the major sources of error likely to be in this experiment? Try and give a numerical estimate to the possible error(s) in the data. Some of the likely sources of error that may occur during this experiment are the calibration process as the hotwire was only calibrated at the centreline and as the hotwire was lowered using screw mechanism which it not totally accurate, there was no calibration o f the at the new position.Another source of error can be due to pressure fluctuations, and due to the velocity being measured using the pressure differences, these fluctuation can cause the velocity to vary. Another source of error could be the assumption of the flow being 2-d as turbulence is a 3-d. To calculate the error, I used the measured velocity table and the theoretical linear calibration velocity. Taking the average error, the percentage error in the experimental data was 5. 8%. Within a range Can not measure supersonic velocities

Friday, November 8, 2019

Definition and Examples of Conjuncts in English Grammar

Definition and Examples of Conjuncts in English Grammar In English grammar, a conjunct, from the Latin, join together, is a word, phrase, or clause linked to another word, phrase, or clause through coordination. For instance, two clauses connected by and (The clown laughed and the child cried) are conjuncts. It may also called a conjoin. The term conjunct can also refer to an adverbial(such as therefore, however, namely) that indicates the relationship in meaning between two independent clauses. The more traditional term for this kind of adverbial is conjunctive adverb. Examples (Definition #1) George and Martha dined alone at Mount Vernon.The back of my head and the head of the bat collided.The dogs barked furiously, and the cat scampered up the tree. Take, for instance, the following sentences from The Revolutionist, [one] of [Ernest] Hemingways short stories [from In Our Time]: He was very shy and quite young and the train men passed him on from one crew to another. He had no money, and they fed him behind the counter in railway eating houses.​​ (Jonathan Cape edn, p. 302) Even in the second sentence, the two clauses which form the conjunct are linked by and, and not, as one might expect in such a discourse context, by so or but. The suppression of complex connectivity in this way seems to have baffled some critics, with comments on the famous Hemingway and ranging from the vague to the nonsensical. (Paul Simpson, Language, Ideology and Point of View. Routledge, 1993) Coordinate Structure Constraint Although a wide variety of structures can be conjoined, not all coordinations are acceptable. One of the first generalizations regarding coordination is Rosss Coordinate Structure Constraint (1967). This constraint states that coordination does not allow for asymmetrical constructions. For example, the sentence This is the man whom Kim likes and Sandy hates Pat is unacceptable, because only the first conjunct is relativized. The sentence This is the man whom Kim likes and Sandy hates is acceptable, because both conjuncts are relativized. . . . Linguists are further concerned with which material is allowed as a conjunct in a coordinate construction. The second example showed conjoined sentences, but coordination is also possible for noun phrases as in the apples and the pears, verb phrases like run fast or jump high and adjectival phrases such as rich and very famous, etc. Both sentences and phrases intuitively form meaningful units within a sentence, called constituents. Subject and verb do not form a constituent in some frameworks of generative grammar. However, they can occur together as a conjunct in the sentence Kim bought, and Sandy sold, three paintings yesterday. (Petra Hendriks, Coordination. Encyclopedia of Linguistics, ed. by Philipp Strazny. Fitzroy Dearborn, 2005) Collective and Average Property Interpretations Consider sentences such as these: The American family used less water this year than last year. The small businessperson in Edmonton paid nearly $30 million in taxes but only made $43,000 in profits last year. The former sentence is ambiguous between the collective and average property interpretations. It could be true that the average American family used less water this year than last while the collective American family used more (due to more families); conversely, it could be true that the average family used more but the collective family used less. As to the latter sentence, which is admittedly somewhat strange (but might be used to further the political interests of Edmonton businesspeople), our world [knowledge] tells us that the first conjunct of the VP must be interpreted as a collective property, since certainly the average businessperson, even in wealthy Edmonton, does not pay $30 million in taxes; but our world knowledge also tells us that the second of the VP conjunctions is to be given an average property interpretation. (Manfred Krifka et al., Genericity: An Introduction. The Generic Book, ed. by Gregory N. Carlson and Francis Jeffry Pelletier. The University of Chicago Pre ss, 1995) Interpreting Naturally and Accidentally Coordinated Noun Phrases [Bernhard] Wlchli ([Co-compounds and Natural Coordination] 2005) discussed two types of coordination: natural and accidental. Natural coordination refers to cases where two conjuncts are semantically closely related (e.g. mum and dad, boys and girls) and are expected to co-occur. On the other hand, accidental coordination refers to cases where the two conjuncts are distant from each other (e.g. boys and chairs, apples and three babies) and are not expected to co-occur. If the two NPs form natural coordination, they tend to be interpreted as a whole. But, if they are accidentally put together, they are interpreted independently. (Jieun Kiaer, Pragmatic Syntax. Bloomsbury, 2014) Declaratives Interrogatives Interestingly, an interrogative main clause can be co-ordinated with a declarative main clause, as we see from sentences like (50) below: (50) [I am feeling thirsty], but [ should I save my last Coke till later]? In (50) we have two (bracketed) main clauses joined together by the co-ordinating conjunction but. The second (italicised) conjunct should I save my last Coke till later? is an interrogative CP [complementiser phrase] containing an inverted auxiliary in the head C position of CP. Given the traditional assumption that only constituents which belong to the same category can be co-ordinated, it follows that the first conjunct I am feeling thirsty must also be a CP; and since it contains no overt complementiser, it must be headed by a null complementiser . . .. (Andrew Radford, An Introduction to English Sentence Structure. Cambridge University Press, 2009) Related Grammar Definitions Compound SentenceConjunction and Coordinating ConjunctionCorrelative Conjunctions

Tuesday, November 5, 2019

The Question Mark

The Question Mark The Question Mark The Question Mark By Sharon The question mark is used at the end of a direct question. Example: What is your name? she asked. It may also be used at the end of a tag question, which changes a statement into a question. Example: He left early, didnt he? Question marks should not be used at the end of indirect questions, such as: I asked my mother whether there were any messages. In a sentence which contains multiple questions, you may include a question mark after each. Example: Who saw the victim last? Her husband? Her son? Her daughter? Question marks are also used to denote missing information. This punctuation mark was first seen in the 8th century and was called the punctus interrogativus. There are many theories about the origin of the symbol, which has changed several times before settling on its current form in the 18th century. The Latin for question was quaestio, which was abbreviated to Qo in the Middle Ages. Its thought that the modern symbol represents the Q placed over the O. The term question mark dates from the 19th century. Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Punctuation category, check our popular posts, or choose a related post below:5 Uses of InfinitivesBody Parts as Tools of Measurement75 Synonyms for â€Å"Hard†

Sunday, November 3, 2019

The Situation Essay Example | Topics and Well Written Essays - 500 words

The Situation - Essay Example According to the constitution, the federal government cannot enter into treaties with an entity, unless it is fully sovereign. The US government between the years 1790 and 1870 has entered into 371 treaties that affirm their sovereignty that is now both inherent and constitutionally valid (Churchill, 1985, p. 31). Throughout history, there have been numerous instances, besides the occupation of their homeland, whereby the government has failed to safeguard the interest of the Native Americans. Furthermore, from an economic point of view, the territories under the Native American tribes are extremely well-endowed with minerals and energy resources. Hence, not only from an ethical viewpoint, the Native American population deserves to enjoy the status of a Nation from a legal and economic perspective as well. The Native population is further divided into three very distinct racial units. Hence essentially there is no all-encompassing term for the numerous racial divisions of the indigenous population of North America (Churchill, 1985, p. 30). Despite the fact that the American constitution has was composed in order to safe guard the interest of every group, but so far it has failed to do anything for the indigenous people or even control the crimes that take place within these tribes. Inherent sovereignty may be a barrier, but it further demonstrates one of the key flaws within the country’s legislative and judicial system that has been unable to reach a position of compromise with the Native American tribesmen and the Government. The situation of the indigenous population is the perfect embodiment of the concept of ‘Internal colonialism’; which is the glaring disparity in development between two regions within the same society. As pointed out and elaborated by Churchill, it is truly a shame that the system fails to protect the rights of the

Friday, November 1, 2019

Summaries Essay Example | Topics and Well Written Essays - 500 words - 1

Summaries - Essay Example Among these sources of threats include malicious codes, industrial espionage, malicious hackers, loss of some physical and infrastructural support, incidences of employee sabotage, fraud and theft, errors and omissions, and threats to personal privacy. A computer virus is a code segment that is capable of replicating by possibly attaching copies of it to existing executable files, implying that viruses can exist in a computer without infecting the system; not unless one opens or runs the malicious program. It is majorly spread by sharing of infected files through emails and removable disks. A worm, on the other hand, is a self-replicating program or algorithm which has the capability of creating copies of it and thereafter executing without the requirement of a host program or user interventions. Just like in the case of viruses, worms exploit the use of network services to propagate itself to other hosts systems within the network topology. A Trojan horse is a program which performs a desired task, however, which also includes the unexpected functions. After installation or running of the Trojan horse, it gets activated and starts to alter the desktops by adding ridiculous active desktop icons; deleting files and destroying other information on the systems; and creating backdoor on the computer systems to offer malicious users the easy lee-ways into the system. Its unique feature that explicitly distinguishes it from worms and viruses are that it does not actually replicate/ reproduce by infecting other files. A Blended threat is rather more sophisticated in the sense that it bundles the worst known features or viruses, worms, Trojan horse and malicious codes. For its aided transmission, it can exploit the server and the linked internet vulnerabilities to initiate, and thereafter transmit by spreading its attacks to other various systems interlinked within the network structure. Blended threats are characterized by